Orthopädische Gelenk-Klinik

Orthopädische Gelenk-Klinik

Alte Bundesstr. 58, 79194 Gundelfingen

Tel: (0761) 55 77 58 0

kontakt@gelenk-doktor.de

Termin anfragen

The Potential of Human Allogeneic Juvenile Chondrocytes for Restoration of Articular Cartilage.

TitelThe Potential of Human Allogeneic Juvenile Chondrocytes for Restoration of Articular Cartilage.
Publication TypeJournal Article
Year of Publication2010
AuthorsAdkisson, DH, Martin, JA, Amendola, RL, Milliman, C, Mauch, KA, Katwal, AB, Seyedin, M, Amendola, A, Buckwalter, JA, Streeter, PR
JournalThe American journal of sports medicine
Date Published2010 Apr 27
SchlüsselwörterKnorpeltransplantation
Zusammenfassung BACKGROUND: Donor-site morbidity, limited numbers of cells, loss of phenotype during ex vivo expansion, and age-related decline in chondrogenic activity present critical obstacles to the use of autologous chondrocyte implantation for cartilage repair. Chondrocytes from juvenile cadaveric donors may represent an alternative to autologous cells. Hypothesis/ PURPOSE: The authors hypothesized that juvenile chondrocyte would show stronger and more stable chondrogenic activity than adult cells in vitro and that juvenile cells pose little risk of immunologic incompatibility in adult hosts. STUDY DESIGN: Controlled laboratory study. METHODS: Cartilage samples were from juvenile (<13 years old) and adult (>13 years old) donors. The chondrogenic activity of freshly isolated human articular chondrocytes and of expanded cells after monolayer culture was measured by proteoglycan assay, gene expression analysis, and histology. Lymphocyte proliferation assays were used to assess immunogenic activity. RESULTS: Proteoglycan content in neocartilage produced by juvenile chondrocytes was 100-fold higher than in neocartilage produced by adult cells. Collagen type II and type IX mRNA in fresh juvenile chondrocytes were 100- and 700-fold higher, respectively, than in adult chondrocytes. The distributions of collagens II and IX were similar in native juvenile cartilage and in neocartilage made by juvenile cells. Juvenile cells grew significantly faster in monolayer cultures than adult cells (P = .002) and proteoglycan levels produced in agarose culture was significantly higher in juvenile cells than in adult cells after multiple passages (P < .001). Juvenile chondrocytes did not stimulate lymphocyte proliferation. CONCLUSION: These results document a dramatic age-related decline in human chondrocyte chondrogenic potential and show that allogeneic juvenile chondrocytes do not stimulate an immunologic response in vivo. CLINICAL RELEVANCE: Juvenile human chondrocytes have greater potential to restore articular cartilage than adult cells, and may be transplanted without the fear of rejection, suggesting a new allogeneic approach to restoring articular cartilage in older individuals.
Full Text
PubMed ID15937706
Folgen Sie der Gelenk-Klinik auf Facebook oder Twitter

Wählen Sie ein Gelenk

Für eine medizinische Fragestellung - Coxarthrose, Arthrose, Gonarthrose, Sprungelenksarthrose, Hüftprothese, Hüftgelenks-TEP, Karpaltunnel-Syndrom, Facettengelenksarthrose etc klicken Sie auf ein Gelenk der orthopädischen Abbildung oben: Sie gelangen dann zu allen wichtigen Informationen Schulter, Sehnenverkalkung, Frozen Shoulder, Auskugelungen, Schultergelenks-Arthrose, Omarthrose, Schleimbeutel, Rotatorenmanschette Taube Hände, Karpaltunnelsyndrom, Medianus Neurolyse minimalinvasiv durchführen Huefte, Coxarthrose, Cox-Arthrose, Hueft-TEP, künstliches Hüftgelenk, Hüftprothese, Hüftschmerzen, Hüftnekrose, Ellenbogen-Gelenk Handgelenk: taube Hände und das Karpaltunnelsyndrom, Medianus Neurolyse minimalinvasiv durchführen knie Haglundferse, Fersensporn, Morton Neurom, Metatarsalgie,  Fuss, Zehen, Hallux Valgus, Hallux Rigidus, Schiefe Zehen, Hammerzehe, Krallenzehe, Korrektur Sprunggelenk, Sprunggelenksprothese, Knöchel, Fussgelenk, Sprunggelenk-Arthrose, Sprunggelenk-Prothese, Sprungelenksarthroskopie, Knorpeltransplantation, Chondrozytentransplantation Wirbelsäule, Bandscheibe, Lendenwirbelsäule, Halswirbelsäule, Bandscheibenvorfall, Rückenschmerzen

MVZ Gelenk-Klinik

Informationen zu Untersuchung und stationärem Aufenthalt in der orthopädischen Gelenk Klinik

MVZ Gelenk-Klinik
EndoProthetikZentrum (EPZ) und Zentrum für Sprung- und Fußgelenk (ZFS)

Spezialisierung:

  • Orthopädie
  • Arthrosetherapie
  • Unfallchirurgie
  • Endoprothesen
  • Neurochirurgie
  • Rehabilitative Medizin

Tel: (0761) 55 77 58 0

praxis@gelenk-doktor.de

Virtueller Rundgang

360° Virtueller Rundgang: Ansicht der Ambulanz